(c) Proline accumulation in wild-type and p5cs1 mutant Arabidopsis plants treated with saline solutions (0.2 M for 2 or 7 days). (d) Calibration curve for semiquantitative analysis of proline content.
pipette up to 50 μl ethanolic extract or 50 μl of 1-0.4-0.2-0.1-0.04 mM proline standard prepared in 70:30 ethanol:water (v/v) seal the tubes, mix and heat at 95°C (either in block heater or ...
Cours sur les techniques d' extraction, de . fractionnement et de purification . Pr. Mohammed Am ine SERGHIN . Université Ibn Zohr. Faculté des Sciences . Département de Biologie. SV3 – M18 – cours sur les techniques d'extraction, de fractionnement et de purification . 1 .
Dosage de la proline (Pro) Le test permet de déterminer la teneur en proline pour les trois traitements (T0, T1, T2) des six variétés de blé dur qui sont effectuées 15 jours après de stress hydrique. ... A. Extraction des échantillons . L'extraction des sucres solubles est faite à froid, en mettant le végétal (prélevé dans le ...
Extraction de la proline des feuilles - Découper à l'aide d'une paire de ciseaux, 100 mg de feuilles fraîchement prélevées ; essai (Figure 1) ; - Ajouter 2 ml de méthanol à 40 % ; - Recouvrir …
Proline and total amino acids may also be extracted using a cold extraction procedure by mixing 20-50 mg fresh weight aliquots with 0.4-1 ml of ethanol:water (40:60 v/v). The resulting mixture is left overnight a 4 ∘ C, and then centrifuged …
Here are some examples of foods that contain proline. • Meat. Proline is found in abundance in meat, including beef, chicken, pork, and lamb. • Dairy Products. Dairy foods such as milk, cheese, and yogurt contain proline. • Eggs, especially egg whites, are a good source of proline. • Fish, such as salmon, tuna, and cod, contain proline.
As a result, the conditions to be used in order to achieve maximum proline extraction with different spectrophotometric methods were determined and optimum values were determined. In addition, since the botanical origin of honey samples significantly affects the proline content of honey, it can be suggested that this study be optimized for ...
m´etabolisme de la proline en absence de contraintes chez A. thaliana (Thiery et al., 2004) tandis que les PLCs, via la signalisation calcique, r´egulent positive-ment la biosynth`ese de la proline lors d'une contrainte saline (Parre et al., 2007). R´ecemment, Ghars et al. (2008a, 2012)ontmisen´evidence l'implication
where Response post-extraction spike is the mean peak area count for the proline spiked into extracted matrix after the extraction procedure, and Response matrix-free spike is for the same concentration of proline in water. Experiments were conducted in triplicate. Accuracy and precision. The intra- or inter-day accuracy and precision of the LC-MS/MS method were …
1 Food and Plant Biology group, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay. ... This chapter summarizes the main strategies for proline extraction and quantification, highlighting their limitations and advantages, and recommends and details a specific protocol for proline ...
Folin-type resins used to purify plant extracts of basic amino acids that interfere with proline determination are not selective and partially retain this amino acid and the basic amino acids. Therefore, their use should be avoided, otherwise correction factors must be introduced. Modifications to another method allow a more rapid and precise estimation of proline.
Avec son design compact et élégant, la centrifugeuse JE400 de proline trouvera facilement sa place sur n'importe quel plan de travail. Performances : Doté d'une puissance de 400 watts, la centrifugeuse de Proline vous permet de préparer des jus délicieux sans effort.
In the determination of proline in honey samples, different analytical methods are used such as fluorimetry (Paramás et al., 2006), liquid chromatography - mass spectrometry (LC-MS) (Rebane & Herodes, 2010), high-resolution liquid chromatography (Ouchemoukh et al., 2010) gas chromatography (GC) (De La Fuente et al., 2011), capillary ...
L'extraction de la proline s'est faite à l'éthanol . selon la méthode de l'AOAC (Association of . Official Analytical Chemists, 1955) modifiée par . Nguyen et Paquin (1971) utilisant .
L'addition de L-proline au milieu de culture (quelles que soient les concentrations testées) stimule la croissance des cultures, même en présence de 200 mM de NaCl. La libération de potassium intracellulaire est inversement corrélée à la …
tendant l'extraction et le dosage de Ia proline. L'extraction at Ia pur~ication de Ia proline sent reali sees a !'ethanol salon Ia methode de I'A.O.A.C. (Asso ciation of Official Analytical Chemists) modifi8e par Nguyen at Paquin (1971). Le dosage a fait appal a Ia technique de Singh et a/. (1973). La densite optique de Ia proline est lue a ...
Abstract Methods are proposed for the extraction of amino acids histidine, methionine, and proline from aqueous media with water-soluble polymers for their subsequent …
L'extraction et le dosage retenus son! decrits dans !'article precedent (Belkhodja et Ait-Sadi, 1993). RESULTATS I - Evolution de Ia proline dans les feuilles, les tiges et les racines au cours du developpement de Ia plante Les figures I, 2 et 3 montrent Ia cineti que d'evolution de Ia proline dans les trois organes de Ia variete locale L (I ...
In the paper there is a formula to convert from your concentrations obtained via the standard curve to µmoles proline/g of fresh weight material: [(µg proline/ml) x ml toluene)/115.5 µg/µmole ...
Méthodes d'extraction et de purification des acides aminés libres et des protéines de tissus végétaux ... an&in&s Acidc aspartique Thrdonine SBrinc Asparagine Acicle' glutamiquc Glutamine Proline Glycinc Alan&' Acicle a-aminobutyriquc Cystinc Valine Mkhionine Mdthionine + mdthionine Isoleucine LFucjnk Tyrosine l?h&nylalaninc Acicle y ...
Proline was the second amino acid synthesized before its presence as a product of the hydrolysis of the protein molecule was established. Willstatter, investigating the position …
II Protocole du dosage de la proline 18 III Protocole du dosage de l'HMF 19 IV Souches testées vis-à-vis des activités antibactériennes des échantillons de miel 22 V Série des dilutions de gradient de deux pour chaque miel 24 VI Spectre pollinique …
Download scientific diagram | Les multiples fonctions de la proline dans les plantes (Szabados et Savouré., 2010). La proline est utilisée pour la synthèse des protéines, elle a des fonctions ...
University of Campania "Luigi Vanvitelli" - 8.776 citazioni - My research interests are primarily centred on the role of compatible osmolytes in stress responses - plant metabolic profiling
Les feuilles de tomate et les disques foliaires sont connus pour accumuler des quantités notables de proline en réponse à la contrainte saline [5], [6].Des expériences préliminaires révèlent cependant que les feuilles des plantes entières de tomate soumises à un stress salin accumulent beaucoup plus de proline que les disques foliaires prélevés sur des …
Significant differences for protein extraction were detected via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). Between the two universal extraction buffers, the USPB showed higher efficiency for extraction of soluble protein, , GR, O2∙-, GPX, SOD, and free proline, while ...
where Response post-extraction spike is the mean peak area count for the proline spiked into extracted matrix after the extraction procedure, and Response matrix-free spike is for the same …
3. Proline - Introduction Proline also protects the structure of membranes and cellular proteins. Many workers have reported several- fold increase in the proline content under physiological and pathological stress conditions. Hence, the analysis of proline is important for assessing the role of proline in physiological and pathological conditions of plants.
In brief, our proline quantification protocol involves reaction of a 1-ml proline sample with 2 ml of 1.25 % ninhydrin in glacial acetic acid at 100 °C for 30 min, followed by recording absorbance of the proline-ninhydrin condensation product in the reaction mixture itself at 508 nm.
ADVERTISEMENTS: In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are …
Proline and total amino acids may also be extracted using a cold extraction procedure by mixing 20-50 mg fresh weight aliquots with 0.4-1 ml of …
These results for cultures of forest species are consistent with findings previously reported for deciduous herbaceous angiosperms and suggest that proline may have a role in protection of forest species from environmental stresses. trees / …
Abstract Methods are proposed for the extraction of amino acids histidine, methionine, and proline from aqueous media with water-soluble polymers for their subsequent determination by capillary electrophoresis. Regularities of the interphase distribution of analytes in water–salt extraction systems based on poly-N-vinyl formamide and its copolymer with N …
to the de novo proline synthesis in plants subjected to water stress, in a. process dependent of ABA concentration. ... Extraction. 4 m L toluene ar e added to each tube, and vortexed f o r 15-20 s.
ADVERTISEMENTS: In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are precipitated as a complex. Other interfering materials are also presumably removed by absorption to the protein-sulphosalicylic acid complex. The extracted proline is made to react with …